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Multivalent HSA Conjugates of 3′‐Sialyllactose are Potent Inhibitors of Adenoviral Cell Attachment and Infection
Author(s) -
Johansson Susanne M. C.,
Arnberg Niklas,
Elofsson Mikael,
Wadell Göran,
Kihlberg Jan
Publication year - 2005
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200400227
Subject(s) - infectivity , virology , serotype , adenovirus infection , chemistry , cell , receptor , virus , biology , biochemistry , microbiology and biotechnology
Adenoviruses of serotypes 8, 19 and 37 are the major cause of the severe eye infection EKC (epidemic keratoconjunctivitis). In general, all adenoviruses interact with their cellular receptors through the fibre proteins, which extend from the virus particle. Recently, adenovirus type 37 (Ad37) was found to bind and infect human corneal cells through attachment to carbohydrate structures that carry terminal α‐(2–3)‐linked sialic acids. Herein we present a synthetic route to a 3′‐sialyllactose derivative and corresponding multivalent HSA conjugates with varying orders of valency. The potential of these compounds as inhibitors of EKC‐causing adenovirus of serotype Ad37, was studied with both a binding assay and an infectivity assay. The results revealed that these compounds effectively prevent Ad37 from binding to and infecting human corneal epithelial (HCE) cells. Moreover, the inhibition is significantly increased with higher orders of multivalency.