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Oligonucleotide Bearing Ethylenediamine‐ N,N,N′ ‐Triacetates for Gap‐Selective DNA Hydrolysis by Ce 4+ /EDTA
Author(s) -
Komiyama Makoto,
Arishima Hiroyuki,
Yokoyama Masahito,
Kitamura Yoshihito,
Yamamoto Yoji
Publication year - 2005
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200400220
Subject(s) - phosphodiester bond , ethylenediamine , bond cleavage , selectivity , hydrolysis , chemistry , oligonucleotide , dna , substrate (aquarium) , cleavage (geology) , stereochemistry , medicinal chemistry , polymer chemistry , inorganic chemistry , organic chemistry , biochemistry , materials science , biology , rna , catalysis , ecology , fracture (geology) , composite material , gene
With the use of two oligonucleotides bearing ethylenediamine‐ N,N,N′ ‐triacetate groups as additives, gap sites were formed at predetermined sites in substrate DNA. Upon treating these systems with a Ce 4+ /EDTA complex at pH 7.0 and 37 °C, the phosphodiester linkages at the gap site were selectively hydrolyzed. The DNA scission was greatly promoted by the introduction of ethylenediaminetriacetate groups, and the scission efficiency increased as the number of these groups increased. Even a one‐base gap was successfully hydrolyzed when three ethylenediaminetriacetate groups were placed consecutively at both edges of the gap, although the scission was minimal in the absence of these groups. The site‐selective scission could be also achieved at higher temperatures without any significant loss of site‐selectivity.