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Chemistry Challenges in SNP Typing
Author(s) -
Nakatani Kazuhiko
Publication year - 2004
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200400161
Subject(s) - genetics , single nucleotide polymorphism , typing , snp , molecular inversion probe , dna , primer extension , base pair , biology , mutant , snp genotyping , computational biology , nucleotide , gene , genotype
Technologies that discriminate between two alleles that differ from each other only in a single base sequence are needed for the typing of single‐nucleotide polymorphisms (SNPs). Hybridization of probe DNAs to wild and mutant targets produces mismatched duplexes, whose chemical properties are clues in chemistry challenges for nonenzymatic SNP typing.