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Adding New Tools to the Arsenal of Expressed Protein Ligation
Author(s) -
Budisa Nediljko
Publication year - 2004
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200400141
Subject(s) - sortase a , ligation , chemical ligation , sortase , native chemical ligation , enzyme , protease , peptide , chemistry , biochemistry , chemical biology , computational biology , combinatorial chemistry , biology , bacterial protein , microbiology and biotechnology , cysteine , gene
In recent years , there has been an increased need for new tools for chemical or enzymatic ligation that allow different protein or peptide fragments to be coupled in a very specific way. Progress has been made by using pure chemical approaches such as native chemical and Staudinger ligations. In parallel, the arsenal of biochemical ligation strategies, including expressed protein and enzyme ligations, has been enriched with novel enzymes such as V8 protease and staphylococcal sortase.