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Fusarin C Biosynthesis in Fusarium moniliforme and Fusarium venenatum
Author(s) -
Song Zhongshu,
Cox Russell J.,
Lazarus Colin M.,
Simpson Thomas J.
Publication year - 2004
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200400138
Subject(s) - biology , polyketide synthase , genomic dna , nonribosomal peptide , complementary dna , fusarium , gene , microbiology and biotechnology , genetics , polyketide , biosynthesis
Fragments of polyketide synthase (PKS) genes were amplified from complementary DNA (cDNA) of the fusarin C producing filamentous fungi Fusarium moniliforme and Fusarium venenatum by using degenerate oligonucleotides designed to select for fungal PKS C ‐methyltransferase ( C MeT) domains. The PCR products, which were highly homologous to fragments of known fungal PKS C MeT domains, were used to probe cDNA and genomic DNA (gDNA) libraries of F. moniliforme and F. venenatum. A 4.0 kb cDNA clone from F. venenatum was isolated and used to prepare a hygromycin‐resistance knockout cassette, which was used to produce a fusarin‐deficient strain of F. venenatum (kb=1000 bp). Similarly, a 26 kb genomic fragment, isolated on two overlapping clones from F. moniliforme , encoded a complete iterative Type I PKS fused to an unusual nonribosomal peptide synthase module. Once again, targeted gene disruption produced a fusarin‐deficient strain, thereby proving that this synthase is responsible for the first steps of fusarin biosynthesis.