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Function of lanGT3 , a Glycosyltransferase Gene Involved in Landomycin A Biosynthesis
Author(s) -
Luzhetskyy Andriy,
Liu Tao,
Fedoryshyn Marta,
Ostash Bohdan,
Fedorenko Viktor,
Rohr Jürgen,
Bechthold Andreas
Publication year - 2004
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200400123
Subject(s) - glycosyltransferase , biosynthesis , mutant , polyketide , gene , biochemistry , trisaccharide , function (biology) , biology , chemistry , genetics
The glycosyltransferase gene lanGT3 , involved in the biosynthesis of the angucyclic antibiotic landomycin A, has been characterised by targeted gene deletion. A lanGT3 mutant was shown to produce landomycin E, which consists of a trisaccharide side chain attached to the polyketide moiety. Expression of lanGT3 in the mutant restored landomycin A production. Our results indicate that LanGT3 is responsible for the transfer of the fourth sugar during landomycin A biosynthesis.