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Identification of the Luciferin–Luciferase System and Quantification of Coelenterazine by Mass Spectrometry in the Deep‐Sea Luminous Ostracod Conchoecia pseudodiscophora
Author(s) -
Oba Yuichi,
Tsuduki Hiroshi,
Kato Shinichi,
Ojika Makoto,
Inouye Satoshi
Publication year - 2004
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200400102
Subject(s) - luciferin , bioluminescence , luciferase , chemistry , luciferases , chemiluminescence , chromatography , biochemistry , transfection , gene
The bioluminescence system of the ostracod Conchoecia pseudodiscophora , which is abundant in the Sea of Japan, has been characterized. The luminescence ( λ max =463 nm) is produced by a luciferin–luciferase reaction, and the luciferin has been identified as coelenterazine. Coelenterazine, coelenteramide, and coelenteramine from C. pseudodiscophora were quantified by LC‐ESI‐MS/MS analysis. The coelenterazine content was estimated to be approximately 230 pg per animal by using a calibration curve of synthetic coelenterazine. The reaction between homogenates of C. pseudodiscophora and synthetic coelenterazine showed luminescence activity; this suggests that a coelenterazine‐type luciferase is present.