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Direct Detection of a Specific Cellular mRNA on Functionalized Microplate
Author(s) -
Alsaidi Mohammed,
Lum Elena,
Huang Zhen
Publication year - 2004
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200400075
Subject(s) - rnase p , rnase h , dna , alkaline phosphatase , chemistry , rna , microbiology and biotechnology , messenger rna , chemiluminescence , polymerase , substrate (aquarium) , enzyme , biochemistry , chromatography , biology , gene , ecology
Detective work . To detect a specific mRNA, a 5′‐DNA‐RNA‐DNA‐3′ template has been designed and immobilized on solid phase, this allows polymerase labeling and extension immediately following RNase H digestion. After alkaline phosphatase labeling and substrate addition, a chemiluminescence single can be detected as per the scheme.