Premium
Dimedone Esters as Novel Hydrolase Substrates and their Application in the Colorimetric Detection of Lipase and Esterase Activity
Author(s) -
Humphrey Cara E.,
Easson Morag A. M.,
Turner Nicholas J.
Publication year - 2004
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200400027
Subject(s) - dimedone , esterase , chemistry , lipase , hydrolase , hydrolysis , chromatography , organic chemistry , enzyme , biochemistry , catalysis
Getting a complex . A novel colorimetric screen has been developed for detecting the activity of lipases and esterases in microtitre‐plate (MTP) format. Hydrolase‐catalyzed hydrolysis of dimedone esters of carboxylic acids yielded dimedone that formed a soluble green complex ( λ max =417 nm) in the presence of copper( II ) acetate and DMSO, see scheme.