Premium
Dimedone Esters as Novel Hydrolase Substrates and their Application in the Colorimetric Detection of Lipase and Esterase Activity
Author(s) -
Humphrey Cara E.,
Easson Morag A. M.,
Turner Nicholas J.
Publication year - 2004
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200400027
Subject(s) - dimedone , esterase , chemistry , lipase , hydrolase , hydrolysis , chromatography , organic chemistry , enzyme , biochemistry , catalysis
Getting a complex . A novel colorimetric screen has been developed for detecting the activity of lipases and esterases in microtitre‐plate (MTP) format. Hydrolase‐catalyzed hydrolysis of dimedone esters of carboxylic acids yielded dimedone that formed a soluble green complex ( λ max =417 nm) in the presence of copper( II ) acetate and DMSO, see scheme.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom