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Engineered Biosynthesis of Phenyl‐Substituted Polyketides
Author(s) -
GarciaBernardo José,
Xiang Longkuan,
Hong Hui,
Moore Bradley S.,
Leadlay Peter F.
Publication year - 2004
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200400007
Subject(s) - polyketide , polyketide synthase , biosynthesis , biochemistry , phenylalanine ammonia lyase , dna ligase , primer (cosmetics) , phenylalanine , chemistry , biology , gene , amino acid , organic chemistry
Polyketide derivatives , such as 1 , are obtained in enhanced yield from added benzoate by coexpressing in Saccharopolyspora erythraea a hybrid erythromycin–soraphen polyketide synthase (PKS) together with benzoate:CoA ligase from the enterocin‐producing ( enc ) pathway of a marine streptomycete. Remarkably, coexpression of the single gene encP encoding phenylalanine ammonia lyase is sufficient to produce the PKS primer benzoyl‐CoA in S. erythraea from endogenous L ‐phenylalanine without any need to supply precursors in the medium.