Mechanistic Implications of  Escherichia coli  Galactokinase Structure‐Based Engineering | Zendy

Hoffmeister Dirk | Zendy; Thorson Jon S. | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

Mechanistic Implications of Escherichia coli Galactokinase Structure‐Based Engineering

Author(s) -

Hoffmeister Dirk,

Thorson Jon S.

Publication year - 2004

Publication title -

chembiochem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.05

H-Index - 126

eISSN - 1439-7633

pISSN - 1439-4227

DOI - 10.1002/cbic.200400003

Subject(s) - galactokinase , escherichia coli , stacking , substrate (aquarium) , protein engineering , chemistry , active site , galactose , hydrogen bond , biochemistry , stereochemistry , biology , enzyme , organic chemistry , molecule , ecology , gene

Engineering GalK : The Escherichia coli galactokinase GalK has been engineered in its active site. The amino acid exchanges Y223F and Y223W broadened the sugar‐substrate spectrum to include, among others, 4‐deoxy‐ D ‐galactose. This finding suggests that the role of Y223 is not primarily hydrogen bonding but rather a hydrophobic stacking to secure the hexopyranose ring in the active site.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research