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Cover Picture: A Fusion of Disciplines: Chemical Approaches to Exploit Fusion Proteins for Functional Genomics (ChemBioChem 9/2003)
Author(s) -
Johnsson Nils,
Johnsson Kai
Publication year - 2003
Publication title -
chembiochem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200390102
Subject(s) - covalent bond , fusion protein , bioorthogonal chemistry , cysteine , dna , chemistry , in vivo , computational biology , biochemistry , linker , proteomics , combinatorial chemistry , biophysics , biology , recombinant dna , click chemistry , gene , genetics , computer science , organic chemistry , enzyme , operating system
The cover picture shows a living cell inside which a protein is labeled with fluorescein. The labeling is based on the unusual mechanism of the DNA repair protein O 6 ‐alkylguanine‐DNA alkyltransferase (AGT, structure on the right), which irreversibly transfers the alkyl group from O 6 ‐alkylguanine to its reactive cysteine residue. By using O 6 ‐benzylguanine derivatives such as the fluorescein derivative shown, AGT fusion proteins can be covalently labeled in vivo. The method is one example of a growing number of approaches that aim at equipping proteins with functionalities that can not be genetically encoded, which opens up new ways to study proteins in vivo. Further information can be found in the article by N. Johnsson and K. Johnsson on p. 803 ff.