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DNA with γ ‐Aminopropyltriethoxysilane Switches between B‐ and C‐Form Structures under Thermal Control
Author(s) -
Yamada Masanori,
Nomizu Motoyoshi,
Satoh Shuya,
Ohkawa Kousaku,
Yamamoto Hiroyuki,
Nishi Norio
Publication year - 2003
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200390038
Subject(s) - dna , ruthenium , molecule , thermostat , a dna , phenanthroline , chemistry , thermal , crystallography , biochemistry , organic chemistry , physics , meteorology , thermodynamics , catalysis
A DNA thermostat : Double‐stranded DNA complexed with γ ‐aminopropyltriethoxysilane (APTES) possesses the B‐form structure at 35 °C and the C‐form structure at 4 °C (see figure). Tris‐(1,10‐phenanthroline)ruthenium( II ) specifically binds to the DNA–APTES complex at 4 °C, which suggests that the DNA structures of the DNA–APTES complex can be switched by thermal control. The DNA–APTES complex could potentially be useful for study of DNA structure as well as in novel materials for optical resolution with thermal control, thermal separation of mutagenic molecules, or thermal sensors.