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Inhibition of Golgi Mannosidase II with Mannostatin A Analogues: Synthesis, Biological Evaluation, and Structure–Activity Relationship Studies
Author(s) -
Li Bing,
Kawatkar Sameer P.,
George Shaji,
Strachan Heather,
Woods Robert J.,
Siriwardena Aloysius,
Moremen Kelley W.,
Boons GeertJan
Publication year - 2004
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200300842
Subject(s) - mannosidase , swainsonine , chemistry , golgi apparatus , stereochemistry , moiety , amine gas treating , docking (animal) , amino acid , molecular model , enzyme , biochemistry , organic chemistry , endoplasmic reticulum , medicine , nursing
Mannostatin and aminocyclopentitetrol analogues with various substitutions at the amino function were synthesized. These compounds were tested as inhibitors of human Golgi and lysosomal α‐mannosidases. Modification of the amine of mannostatin had only marginal effects, whereas similar modifications of aminocyclopentitetrol led to significantly improved inhibitors. Ab initio calculations and molecular docking studies were employed to rationalize the results. It was found that mannostatin and aminocyclopentitretrol could bind to Golgi α‐mannosidase II in a similar mode to that of the known inhibitor swainsonine. However, due to the flexibility of the five‐membered rings of these compounds, additional low‐energy binding modes could be adopted. These binding modes may be relevant for the improved activities of the benzyl‐substituted compounds. The thiomethyl moiety of mannostatin was predicted to make favorable hydrophobic interactions with Arg228 and Tyr727 that would possibly account for its greater inhibitory activity.