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Evaluation of the Molecular Interactions of Fluorinated Amino Acids with Native Polypeptides
Author(s) -
Jäckel Christian,
Seufert Wolfgang,
Thust Sven,
Koksch Beate
Publication year - 2004
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200300840
Subject(s) - amino acid , heptad repeat , fluorine , chemistry , side chain , peptide , coiled coil , stereochemistry , polypeptide chain , combinatorial chemistry , peptide sequence , organic chemistry , biochemistry , gene , polymer
One fluorine can make a difference . Investigation of the properties of fluorinated amino acids in a native‐polypeptide environment increases our ability to use this class of non‐natural amino acids for protein engineering. Two fluorinated amino acids (see figure) were incorporated into different positions of a heptad repeat motif, and their influence on coiled‐coil peptide formation was studied. The screens introduced here are sufficiently sensitive to even detect the difference of one single fluorine atom in the side chain of an amino acid.