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Altering Glucose Oxidase to Oxidize D ‐Galactose through Crosslinking of Imprinted Protein
Author(s) -
Vaidya Alankar,
Borck Alexander,
Manns Anja,
Fischer Lutz
Publication year - 2004
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200300740
Subject(s) - galactose , glucose oxidase , chemistry , aqueous solution , substrate (aquarium) , biochemistry , catalysis , imprinting (psychology) , oxidase test , enzyme , combinatorial chemistry , organic chemistry , gene , biology , ecology
Bioimprinting is established as a potential strategy to improve the catalytic properties of enzymes. The main constraint of this technique is the loss of acquired imprinting property in an aqueous environment. By using a c ross l inked i m p rinting approach (CLIP, see scheme) the substrate spectrum of glucose oxidase was broaden in aqueous solution. Galactose was accepted as a novel substrate by CLIP‐glucose oxidase and it was oxidized to galactono‐1,4‐lactone. Compared with the natural substrate glucose, a surprisingly high 42 % of productivity was reached by CLIP‐glucose oxidase reaction with galactose.