Identification of Luciferyl Adenylate and Luciferyl Coenzyme A Synthesized by Firefly Luciferase | Zendy

Fraga Hugo | Zendy; Esteves da Silva Joaquim C. G. | Zendy; Fontes Rui | Zendy

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Identification of Luciferyl Adenylate and Luciferyl Coenzyme A Synthesized by Firefly Luciferase

Author(s) -

Fraga Hugo,

Esteves da Silva Joaquim C. G.,

Fontes Rui

Publication year - 2004

Publication title -

chembiochem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.05

H-Index - 126

eISSN - 1439-7633

pISSN - 1439-4227

DOI - 10.1002/cbic.200300735

Subject(s) - luciferase , luciferases , bioluminescence , cofactor , luciferin , adenylate kinase , coenzyme a , chemistry , biochemistry , enzyme , transfection , reductase , gene

The firefly luciferase reaction intermediate luciferyl adenylate was detected by RP‐HPLC analysis when the luciferase reaction was performed under a nitrogen atmosphere. Although this compound is always specified as an intermediate in the light‐production reaction, this is the first report of its identification by HPLC in a luciferase assay medium. Under a low‐oxygen atmosphere, luciferase can catalyze the synthesis of luciferyl coenzyme A from luciferin, ATP, and coenzyme A, but in air dehydroluciferyl coenzyme A was produced. The luciferase‐catalyzed synthesis of these coenzyme A derivatives may be a consequence of the postulated recent evolutionary origin of firefly luciferases from an ancestral acyl‐coenzyme A synthetase.

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