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Azide–Alkyne Coupling: A Powerful Reaction for Bioconjugate Chemistry
Author(s) -
Breinbauer Rolf,
Köhn Maja
Publication year - 2003
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200300705
Subject(s) - bioconjugation , cycloaddition , alkyne , click chemistry , chemistry , chemical biology , azide , combinatorial chemistry , coupling reaction , cowpea mosaic virus , nanotechnology , biochemistry , organic chemistry , rna , materials science , gene , catalysis
Tying together biological molecules is a challenge for molecular biologists and chemists. The azide–alkyne [3+2] cycloaddition has recently been introduced as a highly chemoselective ligation reaction in chemical biology. It has found application in the preparation of carbohydrate arrays, in the modification of viruses such as the capsid of the cowpea mosaic virus (CPMV; see scheme), and in the activity‐based protein profiling of whole cells.