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Chemoenzymatic Synthesis of Branched Oligo‐ and Polysaccharides as Potential Substrates for Starch Active Enzymes
Author(s) -
Greffe Lionel,
Jensen Morten T.,
Bosso Claude,
Svensson Birte,
Driguez Hugues
Publication year - 2003
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200300692
Subject(s) - pullulan , amylopectin , chemistry , polysaccharide , glycosyltransferase , enzyme , biochemistry , substrate (aquarium) , starch , stereochemistry , amylose , biology , ecology
Oligo‐ and polysaccharides embodying the α ‐maltotriosyl‐6 II ‐maltotetraosyl structure were readily synthesized by transglycosylation of maltosyl fluoride onto panose and pullulan catalysed by the bacterial transglycosylase cyclodextrin glycosyltransferase (CGTase). The two products obtained proved useful for increasing the knowledge of substrate binding and processing at the active site of barley limit dextrinase that is involved in the metabolism of amylopectin by acting upon its branch points.