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Identification of Peptide Ligands for Target RNA Structures Derived from the HIV‐1 Packaging Signal ψ by Screening Phage‐Displayed Peptide Libraries
Author(s) -
Pustowka Anette,
Dietz Julia,
Ferner Jan,
Baumann Michael,
Landersz Margot,
Königs Christoph,
Schwalbe Harald,
Dietrich Ursula
Publication year - 2003
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200300681
Subject(s) - rna , phage display , peptide , computational biology , biology , human immunodeficiency virus (hiv) , peptide library , chemistry , virology , microbiology and biotechnology , genetics , biochemistry , peptide sequence , gene
Tying up the package : The HIV‐1 RNA packaging signal ψ is a complex RNA structure located within the 5′‐end of unspliced HIV‐1 RNAs (see picture). Upon binding of the viral polyprotein Gag to the ψ RNA, a process mediated by the nucleocapsid protein NCp7, two RNA genomes are packaged into the assembling virions. By using phage display technology peptides were selected that bind specifically to different RNA structures derived from the ψ RNA.