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On Secondary Structure Rearrangements and Equilibria of Small RNAs
Author(s) -
Micura Ronald,
Höbartner Claudia
Publication year - 2003
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200300664
Subject(s) - ribozyme , rna , protein secondary structure , nucleic acid secondary structure , context (archaeology) , nucleic acid structure , nucleotide , computational biology , sequence (biology) , nucleoside , function (biology) , chemistry , biology , stereochemistry , biochemistry , genetics , paleontology , gene
Any way up : Secondary structure ambivalence is encountered even in very small RNAs with only 20–40 nucleotides (see scheme). A combined synthetic and NMR spectroscopic approach allows simple and reliable verification of small RNA secondary structure equilibria. This approach is discussed in the context of RNA conformational transitions, biologically relevant RNA switches, RNA secondary structure changes induced by natural nucleoside modifications, and a structure and function ambivalent ribozyme sequence.