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Gene Expression within Cell‐Sized Lipid Vesicles
Author(s) -
Nomura Shinichiro M.,
Tsumoto Kanta,
Hamada Tsutomu,
Akiyoshi Kazunari,
Nakatani Yoichi,
Yoshikawa Kenichi
Publication year - 2003
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200300630
Subject(s) - vesicle , fluorescence microscope , microreactor , lipid vesicle , green fluorescent protein , gene expression , microbiology and biotechnology , chemistry , fluorescence , cell , protein expression , biophysics , biochemistry , gene , biology , membrane , physics , quantum mechanics , catalysis
Functional protein synthesis was observed in cell‐sized lipid vesicles following encapsulation of a gene‐expression system. Expression of rsGFP (red‐shifted green fluorescent protein) within individual vesicles was observed by fluorescence microscopy. Interestingly, at the early stage of the reaction, the expression efficiency inside the vesicle was remarkably higher than that in the solution outside. The synthesized rsGFP in individual vesicles is safe from attack by proteinase K added to the external aqueous solution. Studies on cell‐sized vesicles expressing protein should contribute to a fundamental understanding of certain aspects of living systems and will be useful for practical applications, such as the construction of microreactors.