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Mirror‐image Phage Display: Aiming at the Mirror
Author(s) -
Wiesehan Katja,
Willbold Dieter
Publication year - 2003
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200300570
Subject(s) - phage display , peptide , mirror image , amino acid , peptide sequence , amino acid residue , chemistry , biophysics , computational biology , biology , biochemistry , physics , optics , gene
Mirror, mirror on the wall, who's the fittest one of all? Peptides are sensitive to proteolytic degradation and may induce immunogenic responses. Mirror‐image phage display circumvents these pitfalls of the common phage‐display technique. Phage‐displayed peptide libraries are screened for peptides that bind to the perfect mirror image of the target molecule, which ultimately yields peptides solely consisting of D ‐amino acid residues that bind to the original target. Such peptides are anticipated to be less sensitive to degradation than L ‐peptides and may be less or not at all immunogenic.