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Glutathione redox system, GSH‐Px activity and lipid peroxidation (LPO) levels in tadpoles of R.r.ridibunda and B.viridis
Author(s) -
Cavas Levent,
Tarhan Leman
Publication year - 2003
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.998
Subject(s) - glutathione , rana ridibunda , lipid peroxidation , chemistry , amphibian , biochemistry , biology , antioxidant , medicine , endocrinology , enzyme , ecology
Total glutathione (t‐GSH), reduced glutathione (GSH), glutathione disulphide (GSSG) levels, t‐GSH/GSSG ratio, glutathione peroxidase (GSH‐Px) activity and lipid peroxidation (LPO) levels were investigated during the development period of a predominantly aquatic amphibian R.r.ridibunda and a predominantly terrestrial amphibian B. viridis. While t‐GSH and GSH showed a similar trend, GSSG concentration increased significantly ( p < 0.05) during the larval stages in R.r.ridibunda larvae. In contrast to R.r.ridibunda larvae, there was no significant ( p >0.05) change between 1 and 5 weeks in the t‐GSH and GSH concentrations of B. viridis. t‐GSH and GSH concentrations of B. viridis larvae became sharply elevated after the fifth week, GSSG levels increased 3.25‐fold during the metamorphosis. The t‐GSH/GSSG ratio fluctuated and the lowest t‐GSH/GSSG ratios were observed at the third week for both species. GSH‐Px activities for both species increased significantly ( p < 0.05) during the growing period. The highest GSH‐Px activities in R.r.ridibunda and B.viridis were observed at the eighth week and they were 3.45 ± 0.17 and 4.1 ± 0.21 IU mg −1 , respectively. The membrane LPO levels in the R.r.ridibunda and B. viridis tadpoles significantly ( p < 0.001) decreased from 206 ± 10.3 to 146 ± 7.3 and from 198 ± 9.9 to 23 ± 1.15 nmol MDA g −1 w.w., respectively. Copyright © 2002 John Wiley & Sons, Ltd.