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Apoptosis induction by S‐allylcysteine, a garlic constituent, during 7,12‐dimethylbenz[a]anthracene‐induced hamster buccal pouch carcinogenesis
Author(s) -
Balasenthil S.,
Rao K. S.,
Nagini S.
Publication year - 2002
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.967
Subject(s) - dmba , 7,12 dimethylbenz[a]anthracene , apoptosis , carcinogenesis , hamster , mesocricetus , golden hamster , chemistry , buccal administration , endocrinology , medicine , biochemistry , biology , pharmacology , gene
The apoptosis‐inducing capacity of S‐allylcysteine (SAC), a water‐soluble garlic constituent, during 7,12‐dimethylbenz[a]anthracene‐induced hamster buccal pouch (HBP) carcinogenesis was investigated in male Syrian hamsters using DNA fragmentation and the apoptosis‐associated proteins, tissue transglutaminase (tTG) and Bcl‐2. Hamsters were divided into four groups of six animals each. Animals in group 1 were painted with a 0.5% solution of DMBA in liquid paraffin on the right buccal pouches three times a week for 14 weeks. Group 2 animals painted with DMBA as in group 1, in addition received 200 mg kg −1 body weight SAC orally on days alternate to DMBA application. Group 3 animals received SAC as in group 2. Group 4 animals received neither DMBA nor SAC and served as the control. The experiment was terminated at the end of 14 weeks. Administration of SAC (200 mg kg −1 body weight) to animals painted with DMBA inhibited DMBA‐induced HBP carcinogenesis as revealed by the absence of neoplasms, induction of tTG and inhibition of Bcl‐2 expression. The results of the present study suggest that SAC may exert its chemopreventive effect by inducing apoptosis. Copyright © 2002 John Wiley & Sons, Ltd.