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Azaanthraquinone inhibits respiration and in vitro growth of long slender bloodstream forms of Trypanosoma congolense
Author(s) -
Nok Andrew Jonathan
Publication year - 2002
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.948
Subject(s) - trypanosoma , mitochondrion , in vitro , dehydrogenase , respiration , biochemistry , biology , in vivo , rotenone , glyceraldehyde 3 phosphate dehydrogenase , enzyme , electron transport chain , virology , anatomy , microbiology and biotechnology
An ethanolic extract of Mitracarpus scaber was found to possess in vitro and in vivo trypanocidal activity against Trypanosoma congolense. At a dosage of 50 mg kg −1 day −1 in normal saline for 5 days, the extract cured Balbc mice infected with T. congolense without any relapse. The isolated active component benz(g)isoquinoline 5,10 dione (Azaanthraquinone) (AQ) purified from the extract was found to inhibit glucose‐dependent cellular respiration and glycerol‐3‐phosphate‐dependent mitochondrial O 2 assimilation of the long bloodstream forms of Trypanosoma congolense. On account of the pattern of inhibition, the target could be the mitochondrial electron transport system composed of glyceraldehyde 3‐phosphate dehydrogenase (G3PDH). The azaanthraquinone specifically inhibited the reduced coenzyme Q 1 ‐dependent O 2 uptake of the mitochondria with respect to ubiquinone. The susceptible site could be due to ubiquinone redox system which links the two enzyme activities. Copyright © 2002 John Wiley & Sons, Ltd.