Premium
Interference of MI‐D, a new mesoionic compound, on artificial and native membranes
Author(s) -
Cadena Silvia M.S.C.,
Carnieri Eva G.S.,
Echevarria Aurea,
de Oliveira Maria Benigna Martinelli
Publication year - 2002
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.932
Subject(s) - mesoionic , valinomycin , membrane fluidity , membrane , chemistry , swelling , diphenylhexatriene , biophysics , membrane potential , nigericin , fluorescence anisotropy , mitochondrion , inner mitochondrial membrane , biochemistry , medicinal chemistry , materials science , biology , composite material
MI‐D (4‐phenyl‐5‐(4‐nitrocinnamoyl)‐1,3,4‐thiadiazolium‐2‐phenylamine chloride), a new mesoionic compound, decreased the rate of swelling induced by valinomycin‐K + , as well as induced swelling in the presence of nigericin‐K + . Shrinkage was also affected, suggesting interference with the inner mitochondrial membrane, which would affect both fluidity and elasticity. Fluorescence polarization of DPH and DPH‐PA, probing the core and outer regions respectively, of the DMPC and native membranes, indicated that MI‐D shifts the midpoint of phase transition to higher values and orders of the fluid phase. These alterations in membrane fluidity are thus related to MI‐D effects on the energy‐linked functions of mitochondria. Copyright © 2001 John Wiley & Sons, Ltd.