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Genetic polymorphism of cytochrome P450 2E1 in the Turkish population
Author(s) -
Ömer Beyhan,
Özbek Uğur,
Akköse Ayfer,
Kiliç Gamze
Publication year - 2001
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.927
Subject(s) - genotype , turkish population , cyp2e1 , genetics , restriction fragment length polymorphism , biology , population , carcinogen , microbiology and biotechnology , polymorphism (computer science) , restriction enzyme , gene , medicine , microsome , environmental health , in vitro
CYP 2E1 is involved in metabolic activation of carcinogenic N‐nitrosamines, benzene, urethane and other low molecular weight compounds. CYP2E1 gene is present in the population in various polymorphic forms. We detected the RFLP of the human CYP2E1 gene with the restriction endonuclease Pst I, Rsa I and Dra I in a group of 153 Turkish individuals. According to the results of the Pst I/ Rsa I analysis, 96.07% of the subjects were of the c1/c1 genotype, and 3.93% were of the c1/c2 genotype. In the Dra I RFLP analysis, 84.30% DD genotype, 15.03% CD genotype and 0.66% CC genotype were determined. The data obtained may be useful in epidemiological studies of the influence of CYP2E1 polymorphism on carcinogenesis. Copyright © 2001 John Wiley & Sons, Ltd.