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3‐Nitrocoumarin is an efficient inhibitor of budding yeast phospholipase‐C
Author(s) -
Tisi Renata,
Coccetti Paola,
Banfi Stefano,
Martegani Enzo
Publication year - 2001
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.918
Subject(s) - yeast , phospholipase , candida albicans , saccharomyces cerevisiae , phospholipase c , biochemistry , biology , phosphatidylinositol , in vitro , chemistry , enzyme , microbiology and biotechnology , signal transduction
3‐Nitrocoumarin is described in the literature as a specific inhibitor of mammalian phospholipase‐C and here we studied the effect of 3‐nitrocoumarin on budding yeast phosphatidylinositol‐specific phospholipase‐C and its effect on yeast growth. 3‐Nitrocoumarin is a powerful inhibitor in vitro of the yeast Plc1 protein with an IC 50 of 57 n m and it is also an inhibitor of yeast growth in minimal media at comparable concentrations. Moreover at the same concentration it inhibits the glucose‐induced PI‐turnover. Since the effects of 3‐nitrocoumarin on yeast growth are superimposable on the growth phenotype caused by PLC1 gene deletion we can conclude that 3‐nitrocoumarin is a specific and selective inhibitor of yeast phospholipase‐C. In addition we show that 3‐nitrocoumarin was also an effective inhibitor of the pathogenic yeast Candida albicans . Copyright © 2001 John Wiley & Sons, Ltd.