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Isotopic methods for probing organization of cellular metabolism
Author(s) -
Sherry A. Dean,
Malloy Craig R.
Publication year - 1996
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.700
Subject(s) - intermediary metabolism , biochemical engineering , cellular metabolism , variety (cybernetics) , computer science , computational biology , nuclear magnetic resonance spectroscopy , biological system , metabolic pathway , metabolism , data science , chemistry , biology , biochemistry , artificial intelligence , engineering , organic chemistry
These examples serve to illustrate that it is now possible to investigate metabolism in intact tissues using a variety of biophysical methods. While we have concentrated on NMR methods, reflecting our own interests in using 13C as a metabolic tracer, GC-mass spectroscopy can often provide similar metabolic information and has the advantage of increased sensitivity over NMR. Combining either or both of these technologies with cleaver 'chemical biopsy' methods offers new opportunities to examine what may seem to be old metabolic questions in a much more relevant environment, the native state.