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Strength exercise suppresses STZ‐induced spatial memory impairment and modulates BDNF/ERK‐CAMKII/CREB signalling pathway in the hippocampus of mice
Author(s) -
Martini Franciele,
Régis Leite Marlon,
Gonçalves Rosa Suzan,
Pregardier Klann Isabella,
Wayne Nogueira Cristina
Publication year - 2020
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.3470
Subject(s) - creb , endocrinology , medicine , tropomyosin receptor kinase b , morris water navigation task , hippocampal formation , mapk/erk pathway , neurotrophic factors , brain derived neurotrophic factor , hippocampus , protein kinase a , chemistry , kinase , receptor , transcription factor , biochemistry , gene
Alzheimer's disease (AD) is a progressive neurodegenerative disorder that has generated scientific interest because of its prevalence in the population. Studies indicate that physical exercise promotes neuroplasticity and improves cognitive function in animal models and in human beings. The aim of the present study was to investigate the effects of strength exercise on the hippocampal protein contents and memory performance in mice subjected to a model of sporadic AD induced by streptozotocin (STZ). Swiss mice received two injections of STZ (3 mg/kg, intracerebroventricular). After 21 days, they began physical training using a ladde. Mice performed this protocol for 4 weeks. After the last exercise training session, mice performed the Morris Water Maze test. The samples of hippocampus were excised and used to determine protein contents of brain‐derived neurotrophic factor (BDNF), extracellular signal‐regulated kinase‐Ca 2+ (ERK), calmodulin‐dependent protein kinase (CAMKII) and cAMP‐response element‐binding protein (CREB) signalling pathway. Strength exercise was effective against the decrease in the time spent and distance travelled in the target quadrant by STZ‐injected mice. Strength exercise was also effective against the reduction of mature BDNF, tropomyosin receptor kinase B and neuronal nuclear antigen (NeuN) hippocampal protein levels in STZ mice. The decrease in the hippocampal ratio of p ERK/ERK, p CAMKII/CAMKII and p CREB/CREB induced by STZ was reversed by strength exercise. Strength exercise decreased Bax/Bcl2 ratio in the hippocampus of STZ‐injected mice. The present study demonstrates that strength exercise modulated the hippocampal BDNF/ERK‐CAMKII/CREB signalling pathway and suppressed STZ‐induced spatial memory impairment in mice.