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Silencing of long noncoding RNA UCA1 inhibits colon cancer invasion, migration and epithelial‐mesenchymal transition and tumour formation by upregulating miR‐185‐5p in vitro and in vivo
Author(s) -
Cao Chen,
Zhang Junhui,
Yang Chuanhua,
Xiang Lili,
Liu Wenneng
Publication year - 2020
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.3454
Subject(s) - gene silencing , gene knockdown , cancer research , colorectal cancer , epithelial–mesenchymal transition , cancer , carcinogenesis , long non coding rna , in vivo , metastasis , small hairpin rna , biology , downregulation and upregulation , cell culture , gene , biochemistry , genetics , microbiology and biotechnology
Colon cancer is the third most common malignancy in the world. Long‐chain noncoding RNA urothelial carcinoma‐associated 1 (UCA1) was abnormally expressed in colon cancer and participated in colon cancer by regulating multiple miRNAs. This study further explored the molecular mechanism of UCA1 in the development of colon cancer from both in vitro and in vivo. The results showed that UCA1 was highly expressed in colon cancer cells, while miR‐185‐5p was low expressed. Bioinformatics analysis showed that miR‐185‐5p was a target of UCA1, while MAPK14 was a target of miR‐185‐5p. Knockdown of UCA1 with shRNA (sh‐UCA1) resulted in a significant increase in miR‐185‐5p and a significant decrease in MAPK14. In addition, sh‐UCA1 inhibited invasion, migration and epithelial‐mesenchymal transformation of colon cancer cells. Western blotting also showed that sh‐UCA1 inactivated the MAPKAPK2/HSP27 pathway. Furthermore, animal studies have revealed that sh‐UCA1 inhibited tumour formation in vivo and improved the survival rate of mice. Collectively, these results suggest that silencing UCA1 may inhibit the carcinogenesis and metastasis of colon cancer in vitro and in vivo by modulating miR‐185‐5p/MAPK14/MAPKAPK2/HSP27 axis. Significance of the study Colon cancer is the third largest malignant tumour worldwide. This study elucidated the role of urothelial carcinoma‐associated 1 (UCA1) in colon cancer cells and its molecular mechanism. The present study suggests that silencing UCA1 may inhibit the invasion, migration, epithelial‐mesenchymal transformation and tumour formation of colon cancer by upregulating miR‐185‐5p in vitro and in vivo. In summary, this study provides a new strategy for targeted therapy of colon cancer.