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Suppression of PRPF4 regulates pluripotency, proliferation, and differentiation in mouse embryonic stem cells
Author(s) -
Park Song,
Han SeHyeon,
Kim HyeonGyeom,
Jeong Jain,
Choi Minjee,
Kim HeeYeon,
Kim MinGi,
Park JinKyu,
Han Jee Eun,
Cho GilJae,
Kim Myoung Ok,
Ryoo Zae Young,
Choi SeongKyoon
Publication year - 2019
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.3437
Subject(s) - gene knockdown , embryoid body , embryonic stem cell , microbiology and biotechnology , biology , germ layer , cellular differentiation , stem cell , chemistry , apoptosis , gene , induced pluripotent stem cell , genetics
Mouse embryonic stem cells (mESCs) are characterized by their self‐renewal and pluripotency and are capable of differentiating into all three germ layers. For this reason, mESCs are considered a very important model for stem cell research and clinical applications in regenerative medicine. The pre‐mRNA processing factor 4 ( PRPF4 ) gene is known to have a major effect on pre‐mRNA splicing and is also known to affect tissue differentiation during development. In this study, we investigated the effects of PRPF4 knockdown on mESCs. First, we allowed mESCs to differentiate naturally and observed a significant decrease in PRPF4 expression during the differentiation process. We then artificially induced the knockdown of PRPF4 in mESCs and observed the changes in the phenotype. When PRPF4 was knocked down, various genes involved in mESC pluripotency showed significantly decreased expression. In addition, mESC proliferation increased abnormally, accompanied by a significant increase in mESC colony size. The formation of mESC embryoid bodies and teratomas was delayed following PRPF4 knockdown. Based on these results, the reduced expression of PRPF4 affects mESC phenotypes and is a key factor in mESC. Significance of the study Our results indicate that PRPF4 affects the properties of mESCs. Suppression of PRPF4 resulted in a decrease in pluripotency of mESC and promoted proliferation. In addition, suppression of PRPF4 also resulted in decreased apoptosis. Moreover, the inhibition of PRPF4 reduced the ability to differentiate and formation of teratoma in mESC. Our results demonstrated that PRPF4 is a key factor of controlling mESC abilities.