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Neuronal‐like differentiated SH‐SY5Y cells adaptation to a mild and transient H 2 O 2 ‐induced oxidative stress
Author(s) -
Akki Rachid,
Siracusa Rosalba,
Morabito Rossana,
Remigante Alessia,
Campolo Michela,
Errami Mohammed,
La Spada Giuseppina,
Cuzzocrea Salvatore,
Marino Angela
Publication year - 2018
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.3317
Subject(s) - oxidative stress , neuroprotection , sh sy5y , apoptosis , chemistry , viability assay , cell , inflammation , microbiology and biotechnology , oxidative phosphorylation , cell culture , biophysics , pharmacology , biochemistry , biology , immunology , genetics , neuroblastoma
Preconditioning (PC) is a cell adaptive response to oxidative stress and, with regard to neurons, can be considered as a neuroprotective strategy. The aim of the present study was to verify how neuronal‐like differentiated SH‐SY5Y cells adapt to a mild and transient H 2 O 2 ‐induced oxidative stress and, hence, whether may be considered as more sensitive cell model to study PC pathways. A first screening allowed to define H 2 O 2 concentrations for PC (10μM‐50μM), applied before damage(100μM H 2 O 2 ). Cell viability measured 24 hours after 100μM H 2 O 2 –induced damage was ameliorated by 24‐hour pre‐exposure to low‐concentration H 2 O 2 (10μM‐30μM) with cell size as well restored. Markers for apoptosis (Bcl‐2 and Bad), inflammation (iNOS), and redox system (MnSOD) were also determined, showing that, in cells pre‐exposed to 10μM H 2 O 2 and then submitted to 100μM H 2 O 2 , Bcl‐2 levels were higher, Bad and iNOS levels were lower than those observed in damaged cells, and MnSOD levels were unchanged. Such findings show that (1) neuronal‐like differentiated SH‐SY5Y cells are a suitable model to investigate PC response and more sensitive to the effect of a mild and transient H 2 O 2 ‐induced oxidative stress with respect to other neuronal cells; (2) 10μM H 2 O 2 –induced PC is mediated by apoptotic and inflammatory pathways, unlike antioxidant system; (3) such neuroprotective strategy and underlying signals proven in neuronal‐like differentiated SH‐SY5Y cells may contribute to understand in vivo PC mechanisms and to define a window for pharmacological intervention, namely, related to ischemic brain damage. Significance of the study Neuronal‐like differentiated SH‐SY5Y cells are a suitable model to investigate PC, an endogenous neuroprotective response to a mild and transient H 2 O 2 ‐induced oxidative stress, elicited by 24‐hour exposure to very low H 2 O 2 concentrations and mediated by both apoptotic and inflammatory pathways. This model reflects in vivo PC mechanisms occurring after brain trauma and provides novel information about pathways and time of protection useful for an appropriate pharmacological intervention.