Premium
Allergen‐specific immune response suppresses interleukin 10 expression in B cells via increasing micro‐RNA‐17‐92 cluster
Author(s) -
Geng XiaoRui,
Qiu ShuQi,
Yang LiTao,
Liu ZhiQiang,
Yang Gui,
Liu JiangQi,
Zeng Lu,
Li XiaoXi,
Mo LiHua,
Liu ZhiGang,
Yang PingChang
Publication year - 2016
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.3207
Subject(s) - immune system , interleukin 4 , microbiology and biotechnology , interleukin , biology , immunology , interleukin 17 , reverse transcription polymerase chain reaction , interleukin 5 , histone deacetylase , messenger rna , chemistry , histone , cytokine , gene , genetics
Interleukin (IL)‐10–expressing B cells play a critical role in the immune homeostasis in the body; its regulation has not been fully understood. Micro‐RNA (miR)‐17‐92 cluster has strong regulation in the immunity. This study tests a hypothesis that miR‐17‐92 cluster suppresses IL‐10 expression in B cells. In this study, peripheral B cells were collected from patients with allergic rhinitis (AR). The B cells were treated with specific allergens, dust mite extracts, in the culture. The expressions of miR‐17‐92 cluster and IL‐10 in the culture were assessed by real‐time quantitative reverse transcription polymerase chain reaction and enzyme‐linked immunosorbent assay. The results showed that the levels of miR‐19a, but not the rest of the 5 members (miR‐17, miR‐18a, miR‐19b, miR‐20a, and miR‐92a), were significantly higher in peripheral B cells from AR patients as than in B cells from healthy participants. Exposure of B cells from AR patients to specific allergen, dust mite extracts, significantly increased the levels if miR‐19a and suppressed the expression of IL‐10 in B cells. The levels of histone deacetylase 11 and acetylated H3K9 were higher, and the RNA polymerase II and c‐Maf (the IL‐10 transcription factor) were lower, at the IL‐10 promoter locus. In conclusion, miR‐19a mediates the allergen‐specific immune response–decreased IL‐10 expression in B cells.