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Preventive effect of Desferal on sperm motility and morphology
Author(s) -
Nenkova Galina,
Stefanov Rossen,
Chervenkov Mihail,
Alexandrova Albena
Publication year - 2016
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.3203
Subject(s) - sperm , chemistry , motility , oxidative stress , lipid peroxidation , sperm motility , incubation , extender , semen , boar , andrology , biochemistry , biophysics , biology , microbiology and biotechnology , medicine , organic chemistry , polyurethane
Transition metal ions, mainly iron, are involved in the generation of highly reactive hydroxyl radicals, which are the most powerful inducers of oxidative damage to all biomolecules. The lipids in sperm membranes are highly susceptible to oxidation. Sperm lipid peroxidation (LPO) leads to decrease of motility and reduction of likelihood for sperm‐oocyte fusion. The excess radical production may affect also the spermatozoa morphology. The aim of the present study was to investigate the effect of Desferal on the LPO, motility, and morphology of boar sperm subjected to oxidative stress. After collection, the ejaculates were equally separated and diluted in a commercial semen extender (experiment 1) or in physiological saline (experiment 2). The ejaculates of the 2 experiments were divided into aliquots, which were incubated with one of the following agents: FeSO 4 (0.1mM), H 2 O 2 (0.5mM), or FeSO 4 + H 2 O 2 (Fenton system), in the presence or absence of Desferal. The application of Desferal in the incubation medium had a protective effect against FeSO 4 + H 2 O 2 ‐induced sperm damage, namely, decrease of LPO; decrease the quantity of immotile spermatozoa and decrease the number of morphological abnormalities, regardless of the used medium. In experiment 2, the presence of FeSO 4 in the incubation medium induced LPO in the same range as the combination FeSO 4 + H 2 O 2 , in which the effect was reduced by Desferal. Thus, the supplement of Desferal to media used for sperm storage and processing could be a useful tool for diminishing oxidative injury and improving the quality of the semen.