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Down‐expression of miR‐154 suppresses tumourigenesis in CD133 + glioblastoma stem cells
Author(s) -
Yang Liang,
Yan Zhongjie,
Wang Yuanyu,
Ma Wandong,
Li Chen
Publication year - 2016
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.3201
Subject(s) - gene knockdown , downregulation and upregulation , glioblastoma , cancer research , cancer stem cell , microrna , stem cell , regulator , u87 , cell culture , biology , cell growth , microbiology and biotechnology , gene , genetics
Glioblastoma multiforme (GBM) is the most common and aggressive form of brain cancer. Evidences have suggested that CD133 is a marker for a subset of glioblastoma cancer stem cells. However, whether miRNA plays a critical role in CD133 + GBM is poorly understood. Here, we identified that miR‐154 was upregulated in CD133 + GBM cell lines. Knockdown of miR‐154 remarkably suppressed proliferation and migration of CD133 + GBM cells. Further study found that PRPS1 was a direct target of miR‐154 in CD133 + GBM cells. Overexpression of PRPS1 exhibited similar effects as miR‐154 knockdown in CD133 + GBMs. Our study identified miR‐154 as a previously unrecognized positive regulator of proliferation and migration in CD133 + GBM cells and a potentially therapeutic target of GBMs. Copyright © 2016 John Wiley & Sons, Ltd.
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