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Protection against oxidative stress by vitamin D in cone cells
Author(s) -
Tohari Ali Mohammad,
Zhou Xinzhi,
Shu Xinhua
Publication year - 2016
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.3167
Subject(s) - oxidative stress , reactive oxygen species , viability assay , antioxidant , oxidative phosphorylation , chemistry , inflammation , vitamin c , vitamin e , vitamin d and neurology , vitamin , microbiology and biotechnology , pharmacology , biology , cell , medicine , endocrinology , biochemistry
Photoreceptor degeneration (PD) refers to a group of heterogeneous outer retinal dystrophies characterized by the death of photoreceptors. Both oxidative stress and inflammation are involved in the pathogenesis of PD. We investigate whether vitamin D has a potential for the treatment of PD by evaluating the anti‐oxidative stress and anti‐inflammatory properties of the active form of vitamin D 3 , 1,α, 25‐dihydroxyvitamin D 3 , in a mouse cone cell line, 661W. Mouse cone cells were treated with H 2 O 2 or a mixture of H 2 O 2 and vitamin D; cell viability was determined. The production of reactive oxygen species (ROS) in treated and untreated cells was measured. The expression of key anti‐oxidative stress and inflammatory genes in treated and untreated cells was determined. Treatment with vitamin D significantly increased cell viability and decreased ROS production in 661W cells under oxidative stress induced by H 2 O 2 . H 2 O 2 treatment in 661W cells can significantly down‐regulate the expression of antioxidant genes and up‐regulate the expression of neurotoxic cytokines. Vitamin D treatment significantly reversed these effects and restored the expression of antioxidant genes. Vitamin D treatment also can block H 2 O 2 induced oxidative damages. The data suggested that vitamin D may offer a therapeutic potential for patients with PD.