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Association of DNA methyltransferases expression with global and gene‐specific DNA methylation in colorectal cancer cells
Author(s) -
Sarabi Mostafa Moradi,
Naghibalhossaini Fakhraddin
Publication year - 2015
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.3126
Subject(s) - dna methylation , methyltransferase , methylation , dnmt1 , cpg site , dna methyltransferase , microbiology and biotechnology , biology , gene expression , epigenetics , gene , genetics
There are conflicting reports regarding the association between DNA methyltransferases (DNMTs) expression and global or gene‐specific DNA methylation in colorectal cancer (CRC) cells. To correlate DNMTs expression with DNA methylation, we quantified DNMT1, DNMT3A and DNMT3B mRNA levels in five CRC cell lines (HCT116, LS180, HT29/219, Caco2 and SW742) by real‐time reverse‐transcriptase polymerase chain reaction (PCR) assay. In addition, we examined the global 5‐methyl cytosine levels and the methylation patterns of 12 CpG islands in these CRC cells by enzyme‐linked immunosorbent assay and methylation‐specific PCR methods, respectively. The average expression levels of three DNMTs in HCT116, Caco2, HT29/219 and SW742, relative to the expression level in LS180 (taken to be 1), were 90·1, 31·6, 2·66 and 1·86. Our data indicated that overall about 1·45%, 1·03%, 0·98%, 0·86% and 0·85% of the cytosines were methylated in the genome of HCT116, Caco2, HT29/219, SW742 and LS180 cells, respectively. The 5‐mC percentages were positively correlated with the relative cellular DNMTs expression in five CRC cell lines as verified by Pearson correlation test. However, we found no positive correlation between mRNA expression of DNMTs and gene promoter hypermethylation in these cells. Our results suggest that cellular DNMT expression is positively correlated with global DNA methylation level but not with regional DNA hypermethylation at each locus. Copyright © 2015 John Wiley & Sons, Ltd.