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Knockdown of NF‐κB p65 subunit expression suppresses growth of nude mouse lung tumour cell xenografts by inhibition of Bcl‐2 apoptotic pathway
Author(s) -
Qu Yun,
Qu Bo,
Wang Xiaofang,
Wu Rong,
Zhang Xiaoye
Publication year - 2015
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.3117
Subject(s) - gene knockdown , tunel assay , apoptosis , small interfering rna , microbiology and biotechnology , cancer research , terminal deoxynucleotidyl transferase , nude mouse , biology , cell growth , cell , chemistry , cell culture , transfection , biochemistry , genetics
Nuclear factor‐kappaB (NF‐κB) is an important transcriptional factor and regulates a variety of pathophysiologic process involved in cell survival and death. This study aimed to assess the effects of NF‐κB p65 subunit knockdown in suppression of nude mouse lung tumour cell xenografts and understands the underlying molecular events. A nude mouse Lewis lung carcinoma cell xenograft model was established, and the mice were intraperitoneally injected with NF‐κB p65 small interfering RNA (siRNA) and sacrificed after 2weeks of tumour cell injection. Tumour xenografts were harvested for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, Western blot and quantitative reverse transcription‐polymerase chain reaction analyses. Results found that compared to the phosphate‐buffered saline (PBS)‐treated or the negative control (NC) siRNA‐treated mice tumour xenograft weight and volume were significantly decreased in the NF‐κB p65 siRNA‐treated mice. The TUNEL positive (apoptosis) cells in xenograft sections were 45 ± 5 in PBS and 38 ± 3 in NC siRNA but increased to 271 ± 11 in p65 siRNA‐treated mice. Compared to the PBS or the NC mice, levels of Bcl‐2 mRNA and protein in tumour xenografts were significantly downregulated in p65 siRNA‐treated mice. Knockdown of NF‐κB p65 subunit expression can significantly inhibit the growth of nude mouse Lewis tumour cell xenografts by inducing tumour cell apoptosis and downregulating pro‐apoptotic protein Bcl‐2 expression. Targeting NF‐κB p65 subunit expression as a potential therapeutic strategy needs further evaluation in treating human lung cancer. Copyright © 2015 John Wiley & Sons, Ltd.