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Inhibitory effects of indole α ‐lipoic acid derivatives on nitric oxide production in LPS/IFNγ activated RAW 264.7 macrophages
Author(s) -
Karabay Arzu Zeynep,
Koc Aslı,
GurkanAlp A. Selen,
Buyukbingol Zeliha,
Buyukbingol Erdem
Publication year - 2015
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.3095
Subject(s) - nitric oxide , lipoic acid , lipopolysaccharide , nitric oxide synthase , chemistry , western blot , biochemistry , antioxidant , nitric acid , pharmacology , biology , immunology , organic chemistry , gene
Alpha‐lipoic acid ( α ‐lipoic acid) is a potent antioxidant compound that has been shown to possess anti‐inflammatory effects. RAW 264.7 macrophages produce various inflammatory mediators such as nitric oxide, IL‐1β, IL‐6 and TNF‐alpha upon activation with LPS ( Lipopolysaccharide) and IFNγ (interferon gamma). In this study, the effect of 12 synthetic indole α ‐lipoic acid derivatives on nitric oxide production and iNOS (inducible nitric oxide synthase) protein expression in LPS/IFNγ activated RAW 264.7 macrophages was determined. Cell proliferation, nitric oxide levels and iNOS protein expression were examined with thiazolyl blue tetrazolium blue test, griess assay and western blot, respectively. Our results showed that all of the indole α ‐lipoic acid derivatives showed significant inhibitory effects on nitric oxide production and iNOS protein levels ( p < 0.05). The most active compounds were identified as compound I‐4b, I‐4e and II‐3b. In conclusion, these indole α ‐lipoic acid derivatives may have the potential for treatment of inflammatory conditions related with high nitric oxide production. Copyright © 2015 John Wiley & Sons, Ltd.