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Diabetic encephalopathy‐related depression: experimental evidence that insulin and clonazepam restore antioxidant status in rat brain
Author(s) -
Wayhs Carlos Alberto Yasin,
Mescka Caroline Paula,
Guerreiro Gilian,
Moraes Tarsila Barros,
Jacques Carlos Eduardo Diaz,
Rosa Andrea Pereira,
Ferri Marcelo Kneib,
Nin Maurício Schüler,
DutraFilho Carlos Severo,
Barros Helena Maria Tannhauser,
Vargas Carmen Regla
Publication year - 2014
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.3076
Subject(s) - oxidative stress , antioxidant , clonazepam , medicine , endocrinology , superoxide dismutase , chemistry , diabetes mellitus , insulin , glutathione peroxidase , glutathione reductase , behavioural despair test , hippocampus , pharmacology , biochemistry , antidepressant
There is increasing evidence suggesting that oxidative stress plays an important role in the development of many chronic and degenerative conditions such as diabetic encephalopathy and depression. Considering that diabetic rats and mice present higher depressive‐like behaviour when submitted to the forced swimming test and that treatment with insulin and/or clonazepam is able to reverse the behavioural changes of the diabetic rats, the present work investigated the antioxidant status, specifically total antioxidant reactivity and antioxidant potential of insulin and clonazepam, as well as the effect of this drugs upon protein oxidative damage and reactive species formation in cortex, hippocampus and striatum from diabetic rats submitted to forced swimming test. It was verified that longer immobility time in diabetic rats and insulin plus clonazepam treatment reversed this depressive‐like behaviour. Moreover, data obtained in this study allowed to demonstrate through different parameters such as protein carbonyl content, 2′7′‐dichlorofluorescein oxidation, catalase, superoxide dismutase, glutathione peroxidase assay, total radical‐trapping antioxidant potential and total antioxidant reactivity that there is oxidative stress in cortex, hippocampus and striatum from diabetic rats under depressive‐like behaviour and highlight the insulin and/or clonazepam effect in these different brain areas, restoring antioxidant status and protein damage. Copyright © 2014 John Wiley & Sons, Ltd.