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Effect of T 3 hormone on neural differentiation of human adipose derived stem cells
Author(s) -
Razavi Shahnaz,
Mostafavi Fatemeh Sadat,
Mardani Mohammad,
Zarkesh Esfahani Hamid,
Kazemi Mohammad,
Esfandiari Ebrahim
Publication year - 2014
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.3074
Subject(s) - adipose tissue , neurosphere , stem cell , nestin , neural stem cell , immunocytochemistry , embryonic stem cell , adult stem cell , mesenchymal stem cell , biology , cellular differentiation , microbiology and biotechnology , stem cell marker , endocrinology , biochemistry , gene
Human adult stem cells, which are capable of self‐renewal and differentiation into other cell types, can be isolated from various tissues. There are no ethical and rejection problems as in the case of embryonic stem cells, so they are a promising source for cell therapy. The human body contains a great amount of adipose tissue that contains high numbers of mesenchymal stem cells. Human adipose‐derived stem cells (hADSCs) could be easily induced to form neuron‐like cells, and because of its availability and abundance, we can use it for clinical cell therapy. On the other hand, T 3 hormone as a known neurotropic factor has important impressions on the nervous system. The aim of this study was to explore the effects of T 3 treatment on neural differentiation of hADSCs. ADSCs were harvested from human adipose tissue, after neurosphere formation, and during final differentiation, treatment with T 3 was performed. Immunocytochemistry, real‐time RT‐PCR, Western blotting techniques were used for detection of nestin, MAP2, and GFAP markers in order to confirm the effects of T 3 on neural differentiation of hADSCs. Our results showed an increase in the number of glial cells but reduction in neuronal cells number fallowing T 3 treatment. Copyright © 2014 John Wiley & Sons, Ltd.