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Effect of vitamin D 3 on behavioural and biochemical parameters in diabetes type 1‐induced rats
Author(s) -
Calgaroto Nicéia Spanholi,
Thomé Gustavo Roberto,
Costa Pauline,
Baldissareli Jucimara,
Hussein Fátima Abdala,
Schmatz Roberta,
Rubin Maribel A.,
Signor Cristiane,
Ribeiro Daniela Aymone,
Carvalho Fabiano Barbosa,
Oliveira Lizielle Souza,
Pereira Luciane Belmonte,
Morsch Vera Maria,
Schetinger Maria Rosa Chitolina
Publication year - 2014
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.3044
Subject(s) - tbars , streptozotocin , diabetes mellitus , endocrinology , medicine , thiobarbituric acid , type 2 diabetes , acetylcholinesterase , chemistry , oxidative stress , enzyme , lipid peroxidation , biochemistry
Diabetes is associated with long‐term complications in the brain and reduced cognitive ability. Vitamin D 3 (VD 3 ) appears to be involved in the amelioration of hyperglycaemia in streptozotocin (STZ)‐induced diabetic rats. Our aim was to analyse the potential of VD 3 in avoiding brain damage through evaluation of acetylcholinesterase (AChE), Na + K + ‐adenosine triphosphatase (ATPase) and delta aminolevulinate dehydratase ( δ ‐ALA‐D) activities and thiobarbituric acid reactive substance (TBARS) levels from cerebral cortex, as well as memory in STZ‐induced diabetic rats. Animals were divided into eight groups ( n = 5): control/saline, control/metformin (Metf), control/VD 3 , control/Metf + VD 3 , diabetic/saline, diabetic/Metf, diabetic/VD 3 and diabetic/Metf + VD 3 . Thirty days after treatment, animals were submitted to contextual fear‐conditioning and open‐field behavioural tests, after which they were sacrificed and the cerebral cortex was dissected. Our results demonstrate a significant memory deficit, an increase in AChE activity and TBARS levels and a decrease in δ ‐ALA‐D and Na + K + ‐ATPase activities in diabetic rats when compared with the controls. Treatment of diabetic rats with Metf and VD 3 prevented the increase in AChE activity when compared with the diabetic/saline group. In treated diabetic rats, the decrease in Na + K + ‐ATPase was reverted when compared with non‐treated rats, but the increase in δ ‐ALA‐D activity was not. VD 3 prevented diabetes‐induced TBARS level and improved memory. Our results show that VD 3 can avoid cognitive deficit through prevention of changes in important enzymes such as Na + K + ‐ATPase and AChE in cerebral cortex in type 1 diabetic rats. Copyright © 2014 John Wiley & Sons, Ltd.