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Uptake and metabolism of d ‐glucose in isolated acinar and ductal cells from rat submandibular glands
Author(s) -
Cetik Sibel,
Rzajeva Aigun,
Hupkens Emeline,
Malaisse Willy J.,
Sener Abdullah
Publication year - 2014
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.3039
Subject(s) - ductal cells , cytochalasin b , submandibular gland , extracellular , medicine , endocrinology , ouabain , metabolism , chemistry , carbohydrate metabolism , glucose transporter , catabolism , glucose uptake , intracellular , biochemistry , biology , insulin , cell , sodium , pancreas , organic chemistry
The present study deals with the possible effects of selected environmental agents upon the uptake and metabolism of d ‐glucose in isolated acinar and ductal cells from the rat submandibular salivary gland. In acinar cells, the uptake of d ‐[U‐ 14 C]glucose and its non‐metabolised analogue 3‐ O ‐[ 14 C‐methyl]‐ d ‐glucose was not affected significantly by phloridzin (0.1 mM) or substitution of extracellular NaCl (115 mM) by an equimolar amount of CsCl, whilst cytochalasin B (20 μM) decreased significantly such an uptake. In ductal cells, both phloridzin and cytochalasin B decreased the uptake of d ‐glucose and 3‐ O ‐methyl‐ d ‐glucose. Although the intracellular space was comparable in acinar and ductal cells, the catabolism of d ‐glucose (2.8 or 8.3 mM) was two to four times higher in ductal cells than in acinar cells. Phloridzin (0.1 mM), ouabain (1.0 mM) and cytochalasin B (20 μM) all impaired d ‐glucose catabolism in ductal cells. Such was also the case in ductal cells incubated in the absence of extracellular Ca 2+ or in media in which NaCl was substituted by CsCl. It is proposed that the ductal cells in the rat submandibular gland are equipped with several systems mediating the insulin‐sensitive, cytochalasin B‐sensitive and phloridzin‐sensitive transport of d ‐glucose across the plasma membrane. Copyright © 2014 John Wiley & Sons, Ltd.