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Monochloramine suppresses the proliferation of colorectal cancer cell line Caco‐2 by both apoptosis and G2/M cell cycle arrest
Author(s) -
Kohda Tetsuya,
Sakuma Satoru,
Abe Muneyuki,
Fujimoto Yohko
Publication year - 2014
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.2992
Subject(s) - apoptosis , cell growth , hypochlorous acid , cell cycle , cell cycle checkpoint , chemistry , flow cytometry , chloramine , oxidative stress , cell culture , colorectal cancer , cancer research , cell , viability assay , caco 2 , microbiology and biotechnology , biochemistry , biology , cancer , chlorine , medicine , genetics , organic chemistry
The aim of this study was to assess a possible role of monochloramine (NH 2 Cl), one of the reactive chlorine species, which induce oxidative stress, on the proliferation of colorectal cancer cell line Caco‐2. At concentrations ranging from 10 to 200 μM, NH 2 Cl (14–61% inhibition), but not hypochlorous acid, dose‐dependently inhibited the cell viability of Caco‐2 cells. Experiments utilizing methionine (a scavenger of NH 2 Cl), taurine‐chloramine and glutamine‐chloramine revealed that only NH 2 Cl affects the cancer cell proliferation among reactive chlorine species, with a relative specificity. Furthermore, flow‐cytometry experiments showed that the anti‐proliferative effect of NH 2 Cl is partially attributable to both apoptosis and G2/M cell cycle arrest. These results suggest that NH 2 Cl has the potential to suppress colorectal cancer cell proliferation. Copyright © 2013 John Wiley & Sons, Ltd.
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