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T‐LAK cell‐originated protein kinase is essential for the proliferation of hepatocellular carcinoma SMMC‐7721 cells
Author(s) -
Chen Fang,
Li Ruwei,
Wang Chenji,
Cao Lihuan,
Wang Yingli,
Yu Long
Publication year - 2013
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.2964
Subject(s) - hepatocellular carcinoma , cell growth , gene knockdown , cancer research , small hairpin rna , cell culture , protein kinase b , cell , cell cycle , protein kinase a , kinase , biology , phosphorylation , microbiology and biotechnology , biochemistry , genetics
Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide and typically has poor prognosis. Like most cancers, altered gene expression was always associated with the induction and maintenance of HCC. Here, we reported that the expression level of T‐LAK cell‐originated protein kinase (TOPK) is significantly up‐regulated in human HCC samples and cell lines. The suppression of TOPK by short hairpin RNA in HCC cell line SMMC‐7721 caused cell cycle arrest and reduced cell growth and colony formation ability. Moreover, the tumor formation ability of the TOPK‐suppression cells was significantly impaired compared with the control cells in nude mice. In addition, the knockdown expression of TOPK reduced the AKT phosphorylation. Taken together, we unveiled a novel role of TOPK which acts as an important positive regulator in human HCC cell proliferation. Copyright © 2013 John Wiley & Sons, Ltd.