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Protective effect of sesaminol from Sesamum indicum Linn. against oxidative damage in PC12 cells
Author(s) -
Cao Wenming,
Dai Mingjun,
Wang Xiang,
Yuan Fengjiao,
Chen Fengxiang,
Zhang Weiyun
Publication year - 2013
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.2935
Subject(s) - catalase , glutathione peroxidase , sesamum , superoxide dismutase , antioxidant , apoptosis , chemistry , glutathione , oxidative stress , oxidative phosphorylation , oxidative damage , intracellular , biochemistry , pharmacology , enzyme , biology , horticulture
Sesaminol is one component of sesame oil and has been widely used as the stabilizer to extend the storage period of food oil in China. In this study, we tried to investigate the antioxidant activity of sesaminol on rat pheochromocytoma (PC12) cells oxidative damaged by H 2 O 2 . Cell viability, LDH level and apoptosis of the PC12 cells were assayed after treatment with sesaminol for 3 h and exposure to H 2 O 2 . Furthermore, superoxide (SOD), catalase (CAT), glutathione peroxidase (GSH‐Px) and intracellular ROS were assayed after exposure of the PC12 cells to H 2 O 2 . The results showed that pre‐treatment with sesaminol prior to H 2 O 2 exposure significantly elevated cell survival rate and SOD, CAT and GSH‐Px activity. Meanwhile, sesaminol declined the secreted LDH level, apoptosis rate and ROS level of H 2 O 2 exposed cells. Thus, sesaminol may protect PC12 against oxidative injury. Copyright © 2012 John Wiley & Sons, Ltd.