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Modulatory effect of methanolic extract of Vernonia amygdalina (MEVA) on tert‐butyl hydroperoxide–induced erythrocyte haemolysis
Author(s) -
Adesanoye Omolola A.,
Molehin Olorunfemi R.,
Delima Adetutu A.,
Adefegha Adeniyi S.,
Farombi Ebenezer O.
Publication year - 2013
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.2933
Subject(s) - haemolysis , vernonia amygdalina , lysis , chemistry , antioxidant , hemolysis , glutathione , lipid peroxidation , reactive oxygen species , in vitro , red blood cell , biochemistry , pharmacology , medicine , biology , immunology , traditional medicine , enzyme
Reactive oxygen species (ROS) have been implicated in the aetiology of several pathological and degenerative diseases. The protective effect of natural products possessing antioxidant properties has played a crucial role in ameliorating these deleterious effects. This study investigated the chemoprotective properties of the methanolic extract of Vernonia amygdalina (MEVA) in an experimental model of tert‐butyl hydroperoxide (t‐BHP)–induced human erythrocyte lysis in vitro . Haemolysis was induced by incubating erythrocytes with t‐BHP (2 and 3 mM) in vitro . Samples of erythrocyte suspensions were removed at different intervals over a 6‐h period, and the degree of haemolysis was measured. The anti‐haemolytic effect of MEVA at 25–150 µg ml –1 concentrations on the samples were assessed and compared with Triton X‐100. Administration of t‐BHP at 2‐ and 3‐mM concentrations significantly ( p < 0.05) induced erythrocyte lysis by 37.5% and 31.4%, respectively. The addition of MEVA, however, reduced t‐BHP–induced erythrocyte lysis significantly ( p < 0.05) by 39.3%, 48.4%, 67.3% and 73.4% at 25, 50, 100 and 150 µg ml –1 concentrations, respectively. MEVA likewise protected against t‐BHP–induced lipid peroxidation significantly ( p < 0.05) at 100 and 150 µg ml –1 by the fourth hour and non‐significantly ( p > 0.05) at all concentrations by the sixth hour. The reduced glutathione level was, however, increased with the administration of t‐BHP, while a delayed addition of MEVA had no protective effect on the t‐BHP–induced cell lysis. These findings therefore suggest that MEVA may have protective antioxidant properties, making it suitable for incorporation into food and drug products. Copyright © 2012 John Wiley & Sons, Ltd.