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Effect of storage time on haematological parameters in mullet, Mugil cephalus
Author(s) -
Faggio C.,
Casella S.,
Arfuso F.,
Marafioti S.,
Piccione G.,
Fazio F.
Publication year - 2013
Publication title -
cell biochemistry and function
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.933
H-Index - 61
eISSN - 1099-0844
pISSN - 0263-6484
DOI - 10.1002/cbf.2915
Subject(s) - mugil , mean corpuscular hemoglobin concentration , mean corpuscular volume , mean corpuscular hemoglobin , hemoglobin , white blood cell , zoology , red blood cell , analysis of variance , biology , physiology , veterinary medicine , fish <actinopterygii> , medicine , fishery , immunology , biochemistry
The aim of the present study was to assess the effect of storage time at +4 °C on red blood cell count (RBC), haematocrit (Hct), haemoglobin (Hb), white blood cell count (WBC), thrombocyte count (TC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) in mullet ( Mugil cephalus ) using an automatic method. After blood collection ( T 0 ), all samples were analyzed using both the manual and automatic method. To test the validation of the automatic method, a paired t ‐test was applied, and no statistical difference was observed. The samples were successively divided into four different aliquots and stored at +4 °C to assess the haematological parameters using the automatic method. The first aliquot was refrigerated for 6 h, the second one for 24 h, the third one for 48 h and the last one at for 72 h. One‐way repeated‐measures ANOVA showed a significant effect of storage time ( P < 0.05) on Hb, WBC, TC, MCH and MCHC. These results suggest that haematological parameters can be assessed within 6 h from blood collection when samples are stored at +4 °C because long‐term storage modifies the results of the analyses. Further studies on these parameters could be still needed in various fish species to validate an appropriate method for haematological analysis useful not only for the evaluation of the health status of animal living in captivity and in aquaculture but also to have reliability environmental haematological biomarkers. Copyright © 2012 John Wiley & Sons, Ltd.